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Pfizer Inc. Associate Scientist, Structural Biology in Boulder, Colorado

The Structural Biology group within Pfizer-Boulder Research and Developmenthas an opening for a junior level biologist. This scientist will beresponsible for maintaining and scaling up (.and.#8805;5L) insect celland E. coli cultures for recombinant protein expression. The individual mayalso be involved in molecular cloning and basic protein analysis andcharacterization. Knowledge in cell biology and experience in handling cellcultures are essential for this role.ROLE RESPONSIBILITIESPassage suspended insect cell cultures.Generate baculovirus stocks for expressing recombinant proteins in insectcells.Evaluate and optimize protein expression in insect cells and E. coli.Multi-liter scale of protein expressions in insect cells and E. coli.Document and maintain records on constructs, strains, and protein expressions.Perform molecular cloning and DNA preparation for transfection.BASIC QUALIFICATIONSBS in Molecular Biology, Cell Biology, Biochemistry or related biologicalscience with experience in handling cell cultures within an industrial oracademic research institute (cell culture experience from a lab course doesnot qualify).Familiar with Microsoft Office.Able to work independently yet cooperatively; enthusiastic about learningnew biology and techniques, and readily apply those learnings to immediatechallenges.PREFERRED QUALIFICATIONSHighly desirable experience includes: E. coli transformation, insect ormammalian cell transfection, and protein expression in fore mentionedhosts, molecular cloning, PCR, gel electrophoresis for DNA and proteinanalysis, and immunoblotting.PHYSICAL/MENTAL REQUIREMENTSAble to lift 50lb objects with dimensions 12 inches in height and 26 inches inwidth.NON-STANDARD WORK SCHEDULE REQUIREMENTSMust have flexible work schedule as some weekend work required.EEO .and. Employment Eligibility:Pfizer is committed to equal opportunity in the terms and conditions ofemployment for all employees and job applicants without regard to race,color, religion, sex, sexual orientation, age, gender identity orgender expression, national origin, disability or veteran status. Pfizeralso complies with all applicable national, state and local laws governingnondiscrimination in employment as well as work authorization and employmenteligibility verification requirements of the Immigration and Nationality Actand IRCA. Pfizer is an E-Verify employer.